J. Strzalka et al., X-ray scattering studies of maquette peptide monolayers. 1. Reflectivity and grazing incidence diffraction at the air/water interface, LANGMUIR, 16(26), 2000, pp. 10404-10418
We present isotherm and X-ray reflectivity (XR) measurements from Langmuir
monolayers of a de novo synthetic di-alpha -helical peptide; consisting of
two identical 31-residue, mostly alpha -helical peptide units joined by a d
isulfide bond at their amino-termini. Fitting the XR data to slab models sh
ows that the dihelices lie in the plane of the interface at low pressures.
The monolayers were insufficiently stable for study at high pressures, but
Langmuir films based on a derivative of the peptide alkylated at its amino
termini permitted investigations over a larger range of pressures. We obser
ved an orientational transition, in which the alpha -helices begin by lying
in the plane of the interface at low surface pressures and orient themselv
es approximately normal to the interface at high pressures. We draw the sam
e conclusions from the XR data when we analyze it using box refinement, an
iterative, model-independent method for recovering structure from XR data.
Mixtures of these palmitoylated peptides with a fatty acid (palmitic acid)
or a phospholipid (DLPE) behaved similarly. None of the systems produced pe
aks in the grazing incidence diffraction signal indicative of long-range or
dering of the upright a-helices. Off-specular in-plane scattering measureme
nts based on the difference signal between the peptide/DLPE mixture and pur
e DLPE suggest that the peptide achieves only liquidlike order within the p
lane. We discuss the implications and prospects for future work on designed
peptide monolayers incorporating prosthetic groups that could be used to s
tudy electron transfer in proteins and provide a basis for biomolecular ele
ctronics applications.