Characterization of porous polylactide foams by image analysis and impedance spectroscopy

The texture of highly porous polylactide foams prepared by freeze-drying of poly-L-lactide (L-PLA) and poly-DL-lactide (DL-PLA) solutions of various c oncentrations was investigated by two novel methodologies, image analysis a nd impedance spectroscopy. Image analysis of scanning electron micrographs of transverse cross-sections at two different magnifications gave informati on on both the macroporosity (1 mum < width 10 <mu>m) and ultramacroporosit y (width > 10 mum). Impedance spectroscopy was used to investigate the tran sport properties of the three-dimensional porous matrices by measurement of ionic conduction. Image analysis showed that: (a) the macroporosity, which mainly contributes to porosity, is independent of the sample composition; (b) when the concentration of the polymer solution is increased, the densit y of the ultramacropores decreases and their average diameter increases; (c ) the distribution of the ultramacropores is more homogeneous in the semicr ystalline L-PLA. foams than in the amorphous DL-PLA counterparts, in which the ultramacropores tend to make clusters. The dielectric properties change d at low frequency, in relation to modifications in the ultramacroporosity. Ultramacropores of the L-PLA foams were found to be more open and more sen sitive to the concentration of the polymer solutions compared with DL-PLA. Expectedly, the mechanical properties of the PLA foams changed with the str ucture of the ultramacroporous network. These results encourage further inv estigations on the texture of porous supports, to collect pertinent informa tion on the physical macro- and ultramacroenvironment in which cells will r eside.