Synthesis and properties of 2 '-deoxycytidine triphosphate carrying c-myc tag sequence

Citation
M. Hinz et al., Synthesis and properties of 2 '-deoxycytidine triphosphate carrying c-myc tag sequence, NUCLEOS NUC, 19(10-12), 2000, pp. 1543-1552
Citations number
7
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
10-12
Year of publication
2000
Pages
1543 - 1552
Database
ISI
SICI code
1525-7770(2000)19:10-12<1543:SAPO2'>2.0.ZU;2-A
Abstract
The synthesis of 2'-deoxycytidine triphosphate carrying mercaptoethyl group s at position 4 of cytosine is described. This nucleoside triphosphate was reacted with a maleimido-peptide carrying the c-mye tag-sequence to yield a peptide-nucleoside triphosphate chimera. Primer extension studies showed t hat the nucleoside triphosphate modified with the peptide sequence is incor porated by DNA polymerases opposite guanine.