D. Loakes et al., Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by dna polymerases., NUCLEOS NUC, 19(10-12), 2000, pp. 1599-1614
We have synthesised and examined the enzymatic incorporation properties of
the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) an
d 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had
hoped would behave as ambivalent base analogues in that they can present tw
o alternative hydrogen-bonding faces either by rotation about the carboxami
de group or about the glycosidic bond. The two pyrrole derivatives, dMTP an
d dDTP, exhibit a preference for incorporation with Klenow polymerase. They
are preferentially incorporated as either A or C.