Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by dna polymerases.

Citation
D. Loakes et al., Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by dna polymerases., NUCLEOS NUC, 19(10-12), 2000, pp. 1599-1614
Citations number
26
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
10-12
Year of publication
2000
Pages
1599 - 1614
Database
ISI
SICI code
1525-7770(2000)19:10-12<1599:SAIOPC>2.0.ZU;2-B
Abstract
We have synthesised and examined the enzymatic incorporation properties of the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) an d 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had hoped would behave as ambivalent base analogues in that they can present tw o alternative hydrogen-bonding faces either by rotation about the carboxami de group or about the glycosidic bond. The two pyrrole derivatives, dMTP an d dDTP, exhibit a preference for incorporation with Klenow polymerase. They are preferentially incorporated as either A or C.