Probing the MVAI methyltransferase region that interacts with DNA: Affinity labeling with the dialdehyde-containing DNA duplexes

Authors
Gritsenko, OM Mikhailov, SN Efimtseva, EV Van Aerschot, A Herdewijn, P Gromova, ES
Citation
Om. Gritsenko et al., Probing the MVAI methyltransferase region that interacts with DNA: Affinity labeling with the dialdehyde-containing DNA duplexes, NUCLEOS NUC, 19(10-12), 2000, pp. 1805-1820
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
10-12
Year of publication
2000
Pages
1805 - 1820
Database
ISI
SICI code
1525-7770(2000)19:10-12<1805:PTMMRT>2.0.ZU;2-8
Abstract
Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxid ized 2'-O-beta -D-ribofuranosylcytidine or 1-(beta -D-galactopyranosyl)thym ine residues was performed. Partial chemical hydrolysis of the covalently b ound methylase in the conjugates with the dialdehyde-containing DNA allowed us to determine the amino acid region in the C terminus of methylase MvaI that interacts with DNA.