Synthesis and RNase L binding and activation of a 2-5A-(5 ')-DNA-(3 ')-PNAchimera, a novel potential antisense molecule

Authors
Verheijen, JC Chen, L Bayly, SF Torrence, PF van der Marel, GA van Boom, JH
Citation
Jc. Verheijen et al., Synthesis and RNase L binding and activation of a 2-5A-(5 ')-DNA-(3 ')-PNAchimera, a novel potential antisense molecule, NUCLEOS NUC, 19(10-12), 2000, pp. 1821-1830
Citations number
26
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
10-12
Year of publication
2000
Pages
1821 - 1830
Database
ISI
SICI code
1525-7770(2000)19:10-12<1821:SARLBA>2.0.ZU;2-K
Abstract
Fully automated solid-phase synthesis gave access to a hybrid in which 5'-p hosphorylated-2'-5'-linked oligoadenylate (2-5A) is connected to the 5'-ter minus of DNA which, in turn, is linked at the 3'-end to PNA [2-5A-(5')-DNA- (3')-PNA chimera]. This novel antisense molecule retains full RNase L activ ation potency while suffering only a slight reduction in binding affinity.