EXTRUDED MICRONUCLEI INDUCED BY COLCHICINE OR ACRYLAMIDE CONTAIN MOSTLY LAGGING CHROMOSOMES IDENTIFIED IN PAINTBRUSH SMEARS BY MINOR AND MAJOR MOUSE DNA PROBES

In the mouse bone marrow micronucleus assay, it was studied whether mi cronuclei (MN) could be expelled from polychromatic erythrocytes (PCE) in a similar way to the main nucleus, To avoid the disrupting centrif ugation step of the conventional bone marrow preparation procedure, th e paintbrush technique was used in the present experiments. With May-G runwald-Giemsa staining of paintbrush slides, 5% of the colchicine (CO L)-induced MN were found attached to the outside membranes of PCE and were regarded as extruded, Of the acrylamide (AA)-induced MN, 22% were extruded, After fluorescence in situ hybridization (FISH) of a total of 300 MN per chemical treatment with the mouse minor and major satell ite DNA probes, 9.7% MN were extruded in the COL group and 8.3% MN wer e extruded in the AA group, FISH showed that 76% of the retained COL-i nduced MN were signal-positive, indicating that they contained entire chromosomes, With AA, 29% minor-positive and 28.3% major-positive reta ined MN were found, confirming its known clastogenicity, However, the observed frequency of signal-positive MN (1.7 MNPCEpos/1000 PCE) in th e AA group was about three times higher than in the control (0.5 MNPCE pos/1000 PCE) which indicates that AA has aneugenic potential, FISH an alysis of the extruded MN showed 72-100% major as well as minor signal s, It is concluded that expelled MN contain mostly entire chromosomes.