HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD WITH ELECTROCHEMICAL DETECTION FOR THE DETERMINATION OF IDRAPRIL, A NOVEL ANGIOTENSIN-CONVERTING ENZYME-INHIBITOR, IN BIOLOGICAL MATRICES

A reversed-phase high-performance liquid chromatographic method for th e determination of idrapril in human and rat plasma and urine and in r at tissue homogenates is described. The method is based on the electro chemical detection of idrapril without prior derivatization. Sample pr eparation simply consists in deproteinization with acetonitrile for pl asma and tissue homogenates and in passage through a Sep-Pak C-18 cart ridge for urine. The limit of quantification is 12.5 ng/ml for plasma, 125 ng/g for tissues and 2.5 mu g/ml for urine. The method is suitabl e for monitoring idrapril plasma pharmacokinetics in humans and its ti ssue distribution and urinary excretion in rats.