Hemoglobin adducts of benzo(a)pyrene in tobacco smokers: Characterization of benzo[a]pyrene adducts in maternal and fetal blood samples

Citation
Sr. Myers et Mt. Pinorini, Hemoglobin adducts of benzo(a)pyrene in tobacco smokers: Characterization of benzo[a]pyrene adducts in maternal and fetal blood samples, POLYCYCL AR, 21(1-4), 2000, pp. 167-186
Citations number
22
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
POLYCYCLIC AROMATIC COMPOUNDS
ISSN journal
10406638 → ACNP
Volume
21
Issue
1-4
Year of publication
2000
Pages
167 - 186
Database
ISI
SICI code
1040-6638(2000)21:1-4<167:HAOBIT>2.0.ZU;2-W
Abstract
The formation of hemoglobin - carcinogen adducts has been detected in carci nogen treated animals and in human populations. Although polynuclear aromat ic hydrocarbons are ubiquitous in the human environment and DNA - aromatic hydrocarbon adducts have been detected in human tissue, the occurrence of h emoglobin - polynuclear aromatic hydrocarbon adducts in humans has not been thoroughly described. In this study we examined the effects of reaction co ndition on the extent of in vitro reaction of human hemoglobin and (+) [H-3 ]benzo[a]pyrene-7,8-diol-9,10-epoxide (anti)(BPDE), a metabolite thought to be largely responsible for the carcinogenic effect of benzo[a]pyrene. The chromatographic properties of the resulting hemoglobin - BPDE adducts were examined by conventional DEAE-cellulose ion exchange liquid chromatography and by reversed phase high performance liquid chromatography. Several adduc ts were formed which were chromatographically resolved from hemoglobin and from the individual globins. We applied these techniques to the qualitative and quantitative assessment of benzo(a)pyrene hemoglobin adducts in smoker s, correlating both maternal and fetal adducts with smoking status.