Sr. Myers et Mt. Pinorini, Hemoglobin adducts of benzo(a)pyrene in tobacco smokers: Characterization of benzo[a]pyrene adducts in maternal and fetal blood samples, POLYCYCL AR, 21(1-4), 2000, pp. 167-186
The formation of hemoglobin - carcinogen adducts has been detected in carci
nogen treated animals and in human populations. Although polynuclear aromat
ic hydrocarbons are ubiquitous in the human environment and DNA - aromatic
hydrocarbon adducts have been detected in human tissue, the occurrence of h
emoglobin - polynuclear aromatic hydrocarbon adducts in humans has not been
thoroughly described. In this study we examined the effects of reaction co
ndition on the extent of in vitro reaction of human hemoglobin and (+) [H-3
]benzo[a]pyrene-7,8-diol-9,10-epoxide (anti)(BPDE), a metabolite thought to
be largely responsible for the carcinogenic effect of benzo[a]pyrene. The
chromatographic properties of the resulting hemoglobin - BPDE adducts were
examined by conventional DEAE-cellulose ion exchange liquid chromatography
and by reversed phase high performance liquid chromatography. Several adduc
ts were formed which were chromatographically resolved from hemoglobin and
from the individual globins. We applied these techniques to the qualitative
and quantitative assessment of benzo(a)pyrene hemoglobin adducts in smoker
s, correlating both maternal and fetal adducts with smoking status.