Transgenic monkeys produced by retroviral gene transfer into mature oocytes

Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral ve ctor into the perivitelline space of 224 mature rhesus oocytes, Later ferti lized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed f or transgene DNA and messenger RNA. ALL tissues that were studied from a fr aternal set of twins, miscarried at 73 days, carried the transgene, as conf irmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.