Sm. Oh et al., Quantitative assessment of estrogenic activity in the water environment ofKorea by the E-SCREEN assay, SCI TOTAL E, 263(1-3), 2000, pp. 161-169
In this study, the E-SCREEN assay was optimized and validated for the sensi
tive quantitative determination of the total estrogenicity in river samples
. River water and sediment samples were collected and analyzed with the E-S
CREEN. River water (101) was extracted using combined solid-phase extractio
n in static adsorption mode with Soxhlet extraction. Estrogenic pollutants
adsorbed to the XAD-4 resin were recovered with 98.24 +/- 5.90% efficiency
by elution with ethyl acetate and dichloromethane (1:9). The detection limi
t by 17 beta -estradiol equivalent concentration (EEQ) of the E-SCREEN assa
y was 8.03 pg EEQ/l. Among the water samples, the estrogenic activity was o
bserved to be higher downstream of the Kumho river (7.43 ng EEQ/l) and upst
ream of Kum river (2.05 ng EEQ/l) than in other samples. More than 3 mg of
equivalent sediment samples from the Kumho river, Kum river and Miho stream
showed partial agonistic effects, and the Mankyung river showed a partial
agonistic effect with only 1.5 mg of sediment. The highest value of RPE was
83.34 downstream of the Kumho river, and the lowest value of RPE was 6.52
downstream of the Miho stream. Full estrogen agonistic activities were obse
rved downstream of the Kumho river and upstream of the Kum river. The parti
al agonistic activity was observed in upstream of the Kumho river, downstre
am of the Mankyung river, and upstream of the Miho stream, and no agonistic
action was observed downstream of the Kum river or Miho stream, or upstrea
m of the Mankyung river. The total estrogenic activity in the river water a
nd sediment samples was between 0.50 pg/L and 7.4 ng/L, 3.39 pg/g and 10.70
pg/g. (C) 2000 Elsevier Science B.V. All rights reserved.