An efficient system has been developed for the in vitro plant regeneration
of Lilium longiflorum Thunb. by culturing receptacle sections from flower b
uds. The sections were cultured on one-half MS medium plus 30 g l(-1) sucro
se, 8 g l(-1) agar, 5.4 muM NAA or 4.9 muM IBA plus 2.2 muM BAP. A section
size of 3-4 mm was found to be optimal. After 60 days an average of 41 shoo
ts were formed per explant. More vigorous shoots were obtained by subcultur
ing on hormone-free medium with 20 g l(-1) sucrose. Rooting occurred on one
-half MS medium with 1.1 muM NAA. Rooted plants were hardened-off in a gree
nhouse for two months, and normal flowering plants were produced. (C) 2001
Elsevier Science B.V. All rights reserved.