Shoot induction and plant regeneration from receptacle tissues of Lilium longiflorum

An efficient system has been developed for the in vitro plant regeneration of Lilium longiflorum Thunb. by culturing receptacle sections from flower b uds. The sections were cultured on one-half MS medium plus 30 g l(-1) sucro se, 8 g l(-1) agar, 5.4 muM NAA or 4.9 muM IBA plus 2.2 muM BAP. A section size of 3-4 mm was found to be optimal. After 60 days an average of 41 shoo ts were formed per explant. More vigorous shoots were obtained by subcultur ing on hormone-free medium with 20 g l(-1) sucrose. Rooting occurred on one -half MS medium with 1.1 muM NAA. Rooted plants were hardened-off in a gree nhouse for two months, and normal flowering plants were produced. (C) 2001 Elsevier Science B.V. All rights reserved.