Different patterns of restriction to B19 parvovirus replication in human blast cell lines

B19 parvovirus can replicate in erythroid progenitor cells and in a small n umber of human blast cell lines. To better understand and analyze the B19 v irus replicative cycle, we performed and compared the infection of bone mar row cells and of different blast cell lines with erythroblastoid and megaka ryoblastoid phenotypic characteristics (UT-7, TF-I, M-07, and B1647). Follo wing in vitro infection, B19-specific nucleic acids were characterized with regard to the genome-replicative intermediates, the transcription pattern, and the localization of virus-specific nucleic acids inside infected cells . While all cell lines tested proved to be susceptible to B19 virus infecti on, two different patterns of restriction to replication of B19 virus were observed. In the first restriction pattern, observed in UT-7 cells, the sin gle-stranded viral DNA was converted to double-stranded replicative interme diates, identical to those found in bone marrow cells, and a full set of vi ral transcripts were observed. However, replication and transcription were restricted to a small subset of cells, and production of capsid proteins wa s not detected. In the second restriction pattern, observed in TF-I, M-07, and B1647 cells, the single-stranded viral DNA was not converted to double- stranded replicative intermediates. (C) 2000 Academic Press.