In vitro induction of HIV-1 replication in resting CD4(+) T cells derived from individuals with undetectable plasma viremia upon stimulation with human T-cell leukemia virus type I

Microbial coinfections have been associated with transient bursts of human immunodeficiency virus (HIV) viremia in patients. In this study we investig ated whether human T-cell leukemia virus type I (HTLV-I), another human ret rovirus that is prevalent among certain HIV-infected populations, can induc e HIV-1 replication in patients who had been successfully treated with high ly active antiretroviral therapy. We demonstrate that supernatants from HTL V-l-producing MT-2 cells can induce in vitro replication of HIV-I from high ly purified, resting CD4(+) T cells obtained from individuals with undetect able plasma viremia. Depletion of proinflammatory cytokines from the supern atants reduced, but did not abrogate, the ability to induce HIV-1 replicati on, indicating that other factors such as HTLV-I Tax or Env also have a rol e. The HTLV-l-mediated effect does not require productive infection: exposu re to heat-inactivated HTLV-I virions, purified Tax protein, or HTLV-I Env glycoprotein also induced expression of HIV-I. Furthermore, we demonstrate that coculture of resting CD4(+) T cells with autologous CD8(+) T cells mar kedly inhibits the HTLV-l-induced virus replication. Our results suggest th at coinfection with HTLV-I may induce viral replication in the latent viral reservoirs; however, CD8(+) T cells may play an important role in controll ing the spread of virus upon microbial stimulation. (C) 2000 Academic Press .