E. Ferrer et al., Efficient transient expression of the beta-glucuronidase reporter gene in garlic (Allium sativum L.), AGRONOMIE, 20(8), 2000, pp. 869-874
A biolistic particle delivery system was used to introduce DNA containing a
P-glucuronidase (gus) reporter gene under the control of the CaMV35S promo
ter in three different garlic (Allium sativum L.) tissues: embryogenic call
i, leaves and basal plate discs. Expression of the reporter gene was assaye
d histochemically and fluorimetrically when the tissues were bombarded with
1 mum diameter gold particles coated with DNA, at a distance of 3 cm from
the stopping plate and using 1100 psi rupture discs. Following bombardment,
high levels of beta -glucuronidase (GUS) were found without the need for t
reatment to block previously reported putative endogenous nuclease activity
.