COMPARATIVE-ANALYSIS OF GENE-EXPRESSION C ODING FOR 2', 5'-OLIGOADENYLATESYNTHETASE AND DSRNA PROTEIN-KINASE IN HUMAN FIBROBLASTS INFECTED WITH SINDBIS VIRUS OR INDUCED WITH ALPHA-INTERFERON

Citation
Tm. Sokolova et al., COMPARATIVE-ANALYSIS OF GENE-EXPRESSION C ODING FOR 2', 5'-OLIGOADENYLATESYNTHETASE AND DSRNA PROTEIN-KINASE IN HUMAN FIBROBLASTS INFECTED WITH SINDBIS VIRUS OR INDUCED WITH ALPHA-INTERFERON, Voprosy virusologii, 42(3), 1997, pp. 98-102
Citations number
31
Categorie Soggetti
Virology
Journal title
ISSN journal
05074088
Volume
42
Issue
3
Year of publication
1997
Pages
98 - 102
Database
ISI
SICI code
0507-4088(1997)42:3<98:COGCOF>2.0.ZU;2-6
Abstract
Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblas t culture following treatment with alpha-IF in a dose of 1000 U or cha llenge. with Karelian lever virus (KFV) (5 PFU/cell, 4 h p. i.) was co mpared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimula ting effect on 2,5-OAS mRNA level, whereas rite virus markedly inhibit ed the synthesis of ds-PK mRNA.