COMPARATIVE-ANALYSIS OF GENE-EXPRESSION C ODING FOR 2', 5'-OLIGOADENYLATESYNTHETASE AND DSRNA PROTEIN-KINASE IN HUMAN FIBROBLASTS INFECTED WITH SINDBIS VIRUS OR INDUCED WITH ALPHA-INTERFERON

Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblas t culture following treatment with alpha-IF in a dose of 1000 U or cha llenge. with Karelian lever virus (KFV) (5 PFU/cell, 4 h p. i.) was co mpared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimula ting effect on 2,5-OAS mRNA level, whereas rite virus markedly inhibit ed the synthesis of ds-PK mRNA.