CULTURING OF T-LYMPHOBLASTOID CELL-LINES IN MEDIA CONTAINING GROWTH PROTEINS

Authors
KORNILAYEVA GV PODCHERNYAYEVA RY MELNICHENKO YI KARAMOV EV
Citation
Gv. Kornilayeva et al., CULTURING OF T-LYMPHOBLASTOID CELL-LINES IN MEDIA CONTAINING GROWTH PROTEINS, Voprosy virusologii, 42(3), 1997, pp. 133-134
Citations number
6
Categorie Soggetti
Virology
Journal title
Voprosy virusologii → ACNP
ISSN journal
05074088
Volume
42
Issue
3
Year of publication
1997
Pages
133 - 134
Database
ISI
SICI code
0507-4088(1997)42:3<133:COTCIM>2.0.ZU;2-Y
Abstract
Two optimal variants of growth medium with different content of growth proteins obtained from cattle and porcine blood sera are recommended for culturing lymphoblastoid cells. Use of growth proteins helps optim ize the conditions of cell culturing by ruling out the toxic effect an d Mycoplasma contamination and ensures the standardization of virologi cal experiments due to the absence of gamma-globulin fraction.