Mih. Helaleh et al., Gas chromatographic-mass spectrometric method for separation and detectionof endocrine disruptors from environmental water samples, ANALYT CHIM, 428(2), 2001, pp. 227-234
The simultaneous determination of endocrine disrupters (phenols) has been d
eveloped using a GC-MS method. Endocrine disrupters in various water sample
s, were extracted using a liquid-liquid extraction process, Acidification p
H 2.0-3.0 and salting (NaBr) of water samples really enhance the extraction
process. The extraction of endocrine disrupters was done and experimented
twice with 50 mi of "dichloromethane". The volume was concentrated from 100
to 1 ml. Internal standard of anthracene was also added to the concentrate
d volumes followed by derivatization with N,O-bis(trimethylsilyl)trifluoroa
cetamide (BSTFA) to form phenol-trimethylsilylate (phenol-TMS) derivative.
The phenol-TMS derivative was analyzed by GC-MS method. The applied concent
rated range was over 10-1000 ng/ml for the total phenols. The recovery test
ranged from 59.5 to 88.6% with relative standard deviations between 1.6 an
d 10.3% of 500 ng/ml of the standard phenols spiked to the water sample and
the detection limit was 1 ng/ml. The proposed method was applied satisfact
ory to the determination of total endocrine disruptors in synthetic mixture
s and different types of water sample, such as river, tap and distilled wat
ers, previously spiked with different amounts of these chemicals. (C) 2001
Elsevier Science B.V. All rights reserved.