Optimisation of sample preparation for the determination of trans-resveratrol and other polyphenolic compounds in wines by high performance liquid chromatography

Different methods for sample preparation as a preliminary stage for the sim ultaneous determination of trans-resveratrol and other polyphenolic compoun ds are compared with the aim to establish the best conditions for the deter mination of these compounds in wine samples by high performance liquid chro matography (HPLC). Sample preparation using liquid-liquid extraction and so lid-phase extraction were compared. Liquid-liquid extraction using as organ ic solvent diethyl ether at pH 2.0 shows the best results. The dried extrac t was solved in methanol/water 1:1 (v/v) that improve drastically the separ ation efficiency. The developed method was applied to the analysis of wines from the Canary Islands. (C) 2001 Elsevier Science B.V. All rights reserve d.