Solid phase microextraction associated with microwave assisted extraction of organochlorine pesticides in medicinal plants

The solid phase microextraction (SPME) associated with microwave assisted e xtraction (MAE) for organochlorine pesticides (OCPs) in medicinal plants we re studied. The influence of SPME extraction time, desorption time and temp erature, and gas chromatography (GC) inlet conditions for the SPME fiber co ated with poly(dimethylsiloxane) (PDMS) were investigated. Extraction time of 90 min, desorption time of 2 min and desorption temperature of 270 degre esC were selected for SPME. Heating time of 10 min and heating power of 80% were the optimum conditions fbr MAE. Three different commonly used medicin al plants were tested including atractylodis rhizoma, glycyrrhizae radix, a nd poria. The associated SPME and MAE using water as the extracting solvent shows good results for the medicinal plants. The method detection limits w ere below 0.13 ng/g using GC electron capture detection. The reproducibilit y of the technique was less than 24% R.S.D. (C) 2001 Elsevier Science B.V. All rights reserved.