2-METHYL-3-OXO-4-PHENYL-2,3-DIHYDROFURAN-2-YL ACETATE - A FLUOROGENICREAGENT FOR DETECTION AND ANALYSIS OF PRIMARY AMINES

Authors
Citation
P. Chen et Mv. Novotny, 2-METHYL-3-OXO-4-PHENYL-2,3-DIHYDROFURAN-2-YL ACETATE - A FLUOROGENICREAGENT FOR DETECTION AND ANALYSIS OF PRIMARY AMINES, Analytical chemistry, 69(14), 1997, pp. 2806-2811
Citations number
30
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032700
Volume
69
Issue
14
Year of publication
1997
Pages
2806 - 2811
Database
ISI
SICI code
0003-2700(1997)69:14<2806:2A-AF>2.0.ZU;2-O
Abstract
A new fluorogenic reagent 2-methyl-3-oxo-4-phenyl-2,3-dihydrofuran-2-y l acetate, has been developed for the analysis of primary amines and a minated carbohydrates by means HPLC, CE, and MALDI/MS. Peptides at 1 p mol (2 x 10(-7) M) levels were successfully labeled and analyzed throu gh CE. The fluorescence derivatives have good stability in both acidic and basic solutions, making their further manipulation and structural analysis possible. The derivatives can be analyzed in reversed-phase HPLC due to the hydrophobic nature of this fluorescent tag. Characteri stic elution intervals between the diastereomeric peaks of the chiral peptide derivatives may be used in structural verification. The labele d peptides and neutral oligosaccharides are also readily detectable th rough MALDI/MS in its positive mode.