IDENTIFICATION OF NATIVE FLAVIN ADDUCTS FROM FUSARIUM-OXYSPORUM USINGACCURATE MASS MATRIX-ASSISTED LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY/

Authors
EDMONDSON RD GADDA G FITZPATRICK PF RUSSELL DH
Citation
Rd. Edmondson et al., IDENTIFICATION OF NATIVE FLAVIN ADDUCTS FROM FUSARIUM-OXYSPORUM USINGACCURATE MASS MATRIX-ASSISTED LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY/, Analytical chemistry, 69(14), 1997, pp. 2862-2865
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
69
Issue
14
Year of publication
1997
Pages
2862 - 2865
Database
ISI
SICI code
0003-2700(1997)69:14<2862:IONFAF>2.0.ZU;2-T
Abstract
Accurate mass matrix-assisted laser desorption/ionization (MALDI) time -of-flight (TOF) mass spectrometry is used to determine the identity o f naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass m easurements (1-2 ppm) combined with an elemental composition search id entifies the unknown FAD substituent as nitrobutane. The use of intern al and external calibration in accurate mass MALDI-TOF is discussed.