Assessment of the pathogenicity of an emu-origin influenza A H5 virus in ostriches (Struthio camelus)

Ostriches were inoculated with a laboratory-derived highly pathogenic avian influenza (HPAI) virus of emu origin, A/emu/TX/39924/93 (H5N2) clone c1B. The aim of this study was to evaluate the pathogenicity of this isolate for ostriches and assess the ability of routine virological and serological te sts to detect infection. Avian influenza virus (AIV) was isolated from cloa cal and tracheal swabs from 2 to 12 days post-infection. AIV was also isola ted from brain, thymus, eyelid, spleen, ovary/testis, liver, air sac, prove ntriculum, duodenum, caecal tonsil, heart, pancreas, kidney, nasal gland an d lung. Virus isolation was also possible from swabs of the luminal surface s of the cloaca, jejunum, lower ileum, bursa of Fabricius, trachea and bone marrow. Birds seroconverted as early as 7 days post-infection. This study suggests that HPAI virus of emu origin replicates extensively in infected o striches without causing significant clinical disease or mortality.