E. Gullberg et al., Expression of specific markers and particle transport in a new human intestinal M-cell model, BIOC BIOP R, 279(3), 2000, pp. 808-813
Citations number
26
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The aim of this work was to establish a new, simplified in vitro model of t
he human M-cell. Cocultures of physically separated human intestinal epithe
lial Caco-2 cells and B-cell lymphoma Raji cells were established. The cocu
ltures were characterized under the criteria of morphology, integrity, expr
ession of M-cell markers and cell adhesion molecules (CAMs), and altered pa
rticle transport, Using this construct, the epithelial cells were transform
ed to cells with an Rf-cell-like morphology and had altered expression of p
otential human M-cell markers (alkaline phosphatase down-regulation and Sia
lyl Lewis A antigen upregulation). The expression of intercellular adhesion
molecule-1 and vascular cell adhesion molecule was altered and there was a
n increased binding of lectins wheat germ agglutinin and peanut agglutinin
with a 40-fold increase in microparticle transport. The particle transport
was size-dependent and could he inhibited at 4 degreesC or by replacing the
Raji B-cells with Jurkat T-cells. This new coculture model will enable con
trolled studies of M-cell development and function in vitro. (C) 2000 Acade
mic Press.