Purification and characterization of nitrite-oxidizing enzyme from heterotrophic Bacillus badius 1-73, with special concern to catalase

Authors
Sakai, K Nisijima, H Ikenaga, Y Wakayama, M Moriguchi, M
Citation
K. Sakai et al., Purification and characterization of nitrite-oxidizing enzyme from heterotrophic Bacillus badius 1-73, with special concern to catalase, BIOS BIOT B, 64(12), 2000, pp. 2727-2730
Citations number
14
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
12
Year of publication
2000
Pages
2727 - 2730
Database
ISI
SICI code
0916-8451(200012)64:12<2727:PACONE>2.0.ZU;2-1
Abstract
Nitrite-oxidizing enzyme I(NiOx I) was purified from a heterotrophic bacter ium, Bacillus badius I-73. The enzyme was a homotetramer of a heme-containi ng peptide, and was similar to catalases from various sources in its N-term inal amino acid sequence. The purified enzyme also catalyzed H2O2 degradati on. The nitrite oxidation reaction required ascorbic acid and oxygen. Succe ssive H2O2 feeding could be substituted for ascorbic acid. These indicated that NiOx I is a catalase and nitrite was oxidized by a peroxidase-like rea ction.