M. Watanabe et al., Novel method for producing hypoallergenic wheat flour by enzymatic fragmentation of the constituent allergens and its application to food processing, BIOS BIOT B, 64(12), 2000, pp. 2663-2667
A novel method is proposed to produce hypoallergenic wheat flour suitable f
or patients allergic to wheat. Wheat flour was mixed with a cellulase solut
ion, and the mixture was incubated at 50 degreesC for 1 h to hydrolyze the
carbohydrate allergens. The hydrolysate was further incubated with actinase
at 40 degreesC for 1 h while gently stirring to decompose the proteinaceou
s allergens. The product was evaluated for its allergenicity by an enzyme-l
inked immunosorbent assay, the results of which suggested negative allergen
icity in most cases. The product changed to a batter state that was difficu
lt to process by the usual methods. Gelatinization of the starch in the pro
duct and the addition of a surfactant were beneficial for food processing.