T. Hoshi et al., Preparation of spatially ordered multilayer thin films of antibody and their binding properties, BIOSENS BIO, 15(11-12), 2000, pp. 623-628
Spatially ordered multilayer thin films containing anti-fluoresceinisothioc
yanate (anti-FITC) were prepared on the surface of a quartz slide to study
the binding properties of the multilayer films. A quartz slide was treated
in solutions of avidin and biotin-labeled anti-FITC alternately and repeate
dly to form multilayer thin films through a strong affinity between avidin
and biotin. A spectrophotometric study revealed explicitly that the thin fi
lms thus prepared consisted of alternate monomolecular layers of avidin and
biotin-labeled anti-FITC. The antibody retained its binding activity to an
tigen in the multilayer thin Nm, though the antigen could not access the an
tibody embedded deep in the multilayer Nm. Only the outermost four or five
layers of antibody were involved in the binding of antigen. (C) 2000 Elsevi
er Science S.A. All rights reserved.