Bacterial epoxide hydrolase-catalyzed resolution of a 2,2-disubstituted oxirane: optimization and upscaling

Authors
Hellstrom, H Steinreiber, A Mayer, SF Faber, K
Citation
H. Hellstrom et al., Bacterial epoxide hydrolase-catalyzed resolution of a 2,2-disubstituted oxirane: optimization and upscaling, BIOTECH LET, 23(3), 2001, pp. 169-173
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
3
Year of publication
2001
Pages
169 - 173
Database
ISI
SICI code
0141-5492(200102)23:3<169:BEHROA>2.0.ZU;2-A
Abstract
The epoxide hydrolase-catalyzed resolution of (+/-)-2-methylglycidyl benzyl ether, a versatile chiral building block for the asymmetric synthesis of b ioactive compounds, mediated by whole cells of Rhodococcus ruber SM 1789 wa s accomplished. Among various parameters (such as temperature, buffer type, pH and catalyst/substrate-ratio) an elevated substrate-concentration prove d to be particularly sensitive with respect to a significant enhancement of the enantioselectivity.