Calcification and degeneration characteristics of the Biocor No-React bovine internal mammary artery (BIMA) - in vivo evaluation in a sheep model

Objective: The No-React process for biomaterials was suggested to prevent c alcification and degeneration. We examined in vivo in a chronic sheep model the performance of a Biocor bovine internal mammary artery (BIMA) graft af ter No-React processing. Methods:'No-React' processing consists of aldehyde cross-linkage following a detoxification process with multiple physical variables and incubation wi th surfactant. Biocor BIMA No-React treated grafts have been interposed in the carotid artery in seven sheep with a Dacron graft as control. Grafts we re explanted after 3 or 6 months and were grossly examined, by X-ray, histo logy, transmission and scanning electron microscopy, and atomic absorption spectrophotometry. Results: In the BIMA, good healing was found with a smooth intimal surface, minimal inflammatory reaction, and a well preserved ultrastructure. Calcif ication increased progressively. In the Dacron grafts, a fibrous covering t he inner surface and infiltration with fibroblasts, histiocytes and macroph ages was noted. Calcium content was stable, Conclusion: Calcification increased progressively in Biocor BIMA No-React g rafts. Minimal inflammatory changes, a smooth inner surface and well preser ved ultrastructure were noted. (C) 2001 The International Society for Cardi ovascular Surgery. Published by Elsevier Science Ltd. All rights reserved.