Delta 32 mutation of the chemokine-receptor 5 gene in inflammatory bowel disease

The gene encoding chemokine receptor 5 (CCR5) is colocalized to the microsa tellite marker D3S1573, which was linked with inflammatory bowel disease. G enetic heterogeneity in inflammatory bowel disease might be defined by a co mbination of the p-ANCA status and immunoregulatory genes. One hundred and twenty healthy unrelated controls, 101 patients with Crohn's disease, and 9 9 patients with ulcerative colitis were genotyped for the Delta 32 mutation of the CCR5 gene. The presence of p-ANCA was determined by the use of indi rect immunofluorescence. After genotyping, patients were stratified accordi ng to p-ANCA status. The frequency of the Delta 32 mutation was not signifi cantly different in controls and patients with Crohn's disease or ulcerativ e colitis (P 0.207 or more). Moreover, the frequency of the mutation was no t significantly different in patients with inflammatory bowel disease after stratification for the p-ANCA status (P 0.482). Regardless of the p-ANCA s tatus, Crohn's disease and ulcerative colitis are not associated with the D elta 32 mutation of the CCR5 gene. (C) 2000 Academic Press.