M. Feili-hariri et Pa. Morel, Phenotypic and functional characteristics of BM-derived DC from NOD and non-diabetes-prone strains, CLIN IMMUNO, 98(1), 2001, pp. 133-142
We compared BM-derived DC from NOD with DC from non-diabetes-prone strains.
NOD myeloid progenitors cultured in GM or GM + IL-4 yielded lower numbers
of DC than similar cultures from other strains. NOD GM + IL-4 DC produced l
ower amounts of IL-12 p70 following CD40 ligation or LPS stimulation in the
presence of IFN-gamma than DC from other strains, although similar levels
of IL-6 and NO were produced by all strains. The low amounts of IL-12 p70 p
roduced by GM + IL-4 DC are despite a more mature DC phenotype observed in
NOD mice. The low DC number could diminish the ability of DC to stimulate i
mmunoregulatory T cells. Furthermore, the differentiation/maturation of DC
from myeloid progenitors is altered, reflecting disorders in the function o
f these cells in NOD BM. (C) 2000 Academic Press.