PCR analysis of cry7 genes in Bacillus thuringiensis by the five conservedblocks of toxins

Authors
Ben-Dov, E Manasherob, R Zaritsky, A Barak, Z Margalith, Y
Citation
E. Ben-dov et al., PCR analysis of cry7 genes in Bacillus thuringiensis by the five conservedblocks of toxins, CURR MICROB, 42(2), 2001, pp. 96-99
Citations number
33
Categorie Soggetti
Microbiology
Journal title
CURRENT MICROBIOLOGY
ISSN journal
03438651 → ACNP
Volume
42
Issue
2
Year of publication
2001
Pages
96 - 99
Database
ISI
SICI code
0343-8651(200102)42:2<96:PAOCGI>2.0.ZU;2-R
Abstract
An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins a nd their encoding DNA sequences. A complete set of five primers was constru cted, four direct and one reverse, yielding four specific amplicons. Modifi ed profiles can identify new cry genes.