The possible involvement of CXCR4 in the inhibition of HIV-1 infection mediated by DP178/gp41

The N-(N36/DP107) and C-terminal peptides (C34/DP178) from two alpha -helic al domains of human immunodeficiency virus type 1 (HIV-1) gp41 inhibited HI V infection. A single-round infection using pseudotyped virus clarified tha t a greater amount of gp41-derived peptides was necessary for the inhibitio n of R5 virus (ADA) infection than for that of X4 virus (LAI) infection. Fu rthermore, R5X4 virus (89.6) infection via CCR5 needs more peptides for inh ibition than its infection via CXCR4 does. A high sensitivity of X4 virus w as partially ascribed to the inhibition of the 12G5 binding to CXCR4 by DP1 78LAI. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.