Genomic Exploration of the Hemiascomycetous Yeasts: 4. The genome of Saccharomyces cerevisiae revisited

Since its completion more than 4 years ago, the sequence of Saccharomyces c ervisiae has been extensively used and studied. The original sequence has r eceived a few corrections, and the identification of genes has been complet ed, thanks in particular to transcriptome analyses and to specialized studi es on introns, tRNA genes, transposons or multigene families. In order to u ndertake the extensive comparative sequence analysis of this program, we ha ve entirely revisited the S. cerevisiae sequence using the same criteria fo r all 16 chromosomes and taking into account publicly available annotations for genes and elements that cannot be predicted. Comparison with the other yeast species of this program indicates the existence of 50 novel genes in segments previously considered as 'intergenic' and suggests extensions for 26 of the previously annotated genes. (C) 2000 Federation of European Bioc hemical Societies. Published by Elsevier Science B.V. All rights reserved.