Genomic Exploration of the Hemiascomycetous Yeasts: 7. Saccharomyces servazzii

The genome of Saccharomyces servazzii was analyzed with 2570 random sequenc e tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 puta tive open reading frames with significant homology to Saccharomyces cervisi ae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S .cerevisiae. About 25% of the S, ser,servazzii genes were identified, assum ing that the gene complement is identical in both yeasts. S. servazii carri es very few transposable elements related to Ty elements in S, cervisiae. M ost of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb, A significant match with the Kluyveromyves lactis linear DNA plasmid pGKL-1 encoded RF4 killer protein s uggests that a related plasmid exists in S, scr,servazzii. The sequences ha ve been deposited with EMBL under the accession numbers AL402279-AL404848. (C) 2000 Federation of European Biochemical Societies. Published by Elsevie r Science B.V. All rights reserved.