Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

The genome of Saccharomyces kluyveri was explored through 2528 random seque nce tags with an average length of 981 bp, The complete nuclear ribosomal D IVA unit was found to be 8656 bp in length. Sequences homologous to retroel ements of the gypsy and copier types were identified as well as numerous so lo long terminal repeats. We identified at least 1406 genes homologous to S accharomyces cerevisiae open reading frames, with on average 58.1%, and 72. 4%, amino acid identity and similarity, respectively. In addition, by compa rison with completely sequenced genomes and the SwissProt database, we foun d 27 no, el S. kluyveri genes. Most of these genes belong to pathways or ha ve functions absent from S. cervisiae, such as the catabolic pathway of pur ines or pyrimidines, melibiose fermentation, sorbitol utilization, or degra dation of pollutants, The sequences are deposited in EMBL under the accessi on numbers AL404849-AL407376, (C) 2000 Federation of European Biochemical S ocieties. Published by Elsevier Science B.V. All rights reserved.