Genomic Exploration of the Hemiascomycetous Yeasts: 10. Kluyveromyces thermotolerans

A genomic exploration of Kluyveromyces thermo-tolerans was performed by ran dom sequence tag (RST) analysis. We sequenced 2653 RSTs corresponding to in serts sequenced from both ends. We performed a systematic comparison with a complete set of proteins from Saccharomyces cervisiae, other completely se quenced genomes and SwissProt, We identified six mitochondrial genes and 13 58-1496 nuclear genes by comparison with S.cervisiae. In addition, 25 genes were identified by comparison with other organisms, This corresponds to ab out 24%;, of the estimated gene content of this organism. A lower level of conservation is observed with orthologues to genes of S., cerevisiae previo usly classified as orphans. Gene order was found to be conserved between S. cervisiae and K. thermotolerans in 56.%, of studied cases.(C) 2000 Federat ion of European Biochemical Societies. Published by Elsevier Science B.V. A ll rights reserved.