Regulated production of interferon-inducible T-cell chemoattractants by human intestinal epithelial cells

Background & Aims: Human intestinal epithelial cells inducibly express neut rophil and monocyte chemoattractants, yet little is known about the regulat ed production of T-cell chemoattractants by the intestinal epithelium. IP-1 0, Mig, and I-TAG are 3 CXC chemokines that are known to act as CD4(+) T-ce ll chemoattractants, Methods: We studied constitutive chemokine expression in human colon, and defined the regulated expression of these chemokines by reverse-transcription polymerase chain reaction, enzyme-linked immunosorbe nt assay, and immunohistology using cultured human intestinal epithelial ce ll lines and a novel adaptation of an in vivo human intestinal xenograft mo del. Results: IP-10 and Mig were constitutively expressed by normal human c olon epithelium, and their cognate receptor, CXCR3, was expressed by mucosa l mononuclear cells. Interferon (IFN)-gamma stimulation increased mRNA expr ession and the polarized basolateral secretion of these chemokines by human colon epithelial cell lines; infection with enteroinvasive bacteria, or st imulation with the proinflammatory cytokines tumor necrosis factor or and i nterleukin la, strongly potentiated IFN-gamma -induced epithelial cell IP-1 0, Mig, and I-TAG production. Epithelial cell mRNA and protein expression o f IP-10, Mig and I-TAG were rapidly up-regulated in human intestinal xenogr afts in response to stimulation with IFN-gamma alone or in combination with IL-1, Conclusions: The constitutive and regulated production of the IFN-ga mma -inducible chemokines IP-10, Mig, and I-TAG by human intestinal epithel ium, and the expression of their cognate receptor, CXCR3, by mucosal mononu clear cells, suggest that the intestinal epithelium can play a role in modu lating physiologic and pathologic T cell-mediated mucosal inflammation.