Adenoviral vector design for high-level transgene expression in primitive human hematopoietic progenitors

Adenoviral vector-mediated transient gene expression can provide new possib ilities for ex vivo manipulation of quiescent hematopoietic stem cells (HSC ). In order to define a suitable expression cassette for high levels of tra nsgene expression in HSCs, we have studied the level of transgene expressio n in human CD34(+)CD38(-) cells using adenoviral vectors with various gene expression cassettes encoding the enhanced green fluorescence protein (EGFP ) gene. CD34(+) hematopoietic cells were cultured in serum-free medium with megakaryocyte growth and development factor (MGDF) alone for supporting th e survival of primitive progenitors or with MGDF, c-kit ligand (KL) and flt 3 ligand (FL) for inducing proliferation of primitive progenitors. With all the vectors tested, higher percentages of EGFP expressing cells were found in CD34(+)CD38(-) cells than those in CD34(+)CD38(high) cells from all don ors tested. The phosphoglycerate kinase (PGK)-1 promoter was found to allow higher levels of EGFP expression than the human cytomegalovirus (HCMV) pro moter in CD34(+) CD38 cells. Replacing the SV40 polyadenylation signal with the human beta -globin gene IVS2 and polyadenylation signal in the express ion cassette (AdSxPGK-EGFP-beta -globin) enhanced the level of EGFP express ion markedly further. These results provide a guideline for the development of adenoviral vectors for gene expression in human primitive hematopoietic progenitor cells.