Al. Kistler et C. Guthrie, Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase, GENE DEV, 15(1), 2001, pp. 42-49
Mammalian U2AF65 and UAP56 are required for prespliceosome (PS) formation.
We tested the predictions that the yeast UAP56 homolog, SUBS is required fo
r the same step and functions collaboratively with MUDS the yeast homolog o
f U2AF65. Unexpectedly, sub2-1. extracts accumulate PS-like complexes. More
over, deletion of MUD2 exacerbates the cs phenotype of sub2 alleles yet sup
presses both the is sub2-1 and the lethal Delta sub2 phenotypes. We propose
that Sub2 functionally interacts with Mud2 both before and after PS format
ion. In the absence of Mud2, Sub2 function becomes dispensable.