Recently the platelet and T-cell activation antigen 1 (PTA1) was assigned a
s CD226 at the 7th Conference and Workshop on Human Leukocyte Differentiati
on antigens (HLDA). PTA1 is mainly expressed on activated T cells, natural
killer (NK) cells, platelets and stimulated endotheliocytes, and involved i
n the differentiation of cytotoxic T lymphocytes (CTL) and NK, as well as p
latelet activation and aggregation. We raised hybridomas secreting monoclon
al antibodies (MAbs) to PTA1 by using the natural PTA1 as immunogen, which
was purified from platelets via affinity chromatography. These MAbs, design
ated FMU1, FMU2, FMU3, FMU4, FMU5, FMU6 and FMU7, could recognize PTA1 cDNA
transfected COS7 cells detected by flow cytometry (FCM), and also react wi
th both natural PTA1 and PTA1/Ig fusion protein in indirect enzyme-linked i
mmunoadsorbent assay (ELISA). The biosensor epitope mapping assay showed th
at the seven MAbs, together with previous PTA1-specific MAbs Leo A1 and New
E1, could bind seven distinct epitopes of PTA1, respectively. The panel of
MAbs might be new powerful tools to study the structure-function relations
hip of PTA1 molecule, and to search for the ligand of PTA1.