Evaluation of myc and chromosome 8 copy number in colorectal cancer using interphase cytogenetics

To reveal the significance of genetic abnormalities of the c-myc gene, 56 c olorectal tumors (43 colorectal carcinomas, 5 recurrent or metastatic tumor s, and 8 adenomatous polyps) were analyzed using fluorescence in situ hybri dization (FISH). Two probes specific for c-myc and the chromosome 8 centrom ere were used for dual color FISH. In each case, 100-200 nuclei were observ ed for signals from the probes. The percent of nuclei with c-myc amplificat ion (PMA) was defined as the proportion of nuclei representing the ratio of c-myc/chromosome 8 >1.0, and the percent of nuclei with the greater number of c-myc (PGNM) was defined as the proportion of nuclei representing the r atio of c-myc/chromosome 8 greater than or equal to2.0. Low level amplifica tion was defined as a case with PMA greater than or equal to 10% and PGNM < 10%. High level amplification was defined as a case with PGNM <greater than or equal to>10%. While adenomatous polyps and in situ carcinomas showed no c-myc amplification, the low level amplification and high level amplificat ion of c-myc were observed in 48.8% (21/43) and 20.9% (9/43) of primary col orectal carcinomas. In addition, the group including cases of stage IIIb an d IV exhibited significantly higher average copy numbers of c-myc (CN-myc), PMA and PGNM than the other group of earlier stages. FISH was thought a us eful cytogenetic method to detect genetic abnormalities in solid tumors. It was shown that the c-myc gene amplification identified using FISH was asso ciated with the aggressiveness of colorectal carcinoma.