Identification of a genomic island present in the majority of pathogenic isolates of Pseudomonas aeruginosa

Pseudomonas aeruginosa, a ubiquitous gram-negative bacterium, is capable of colonizing a wide range of environmental niches and can also cause serious infections in humans, In order to understand the genetic makeup of pathoge nic P. aeruginosa strains, a method of differential hybridization of arraye d libraries of cloned DNA fragments was developed, An M13 library of DNA fr om strain X24509, isolated from a patient with a urinary tract infection, w as screened using a DNA probe from P. aeruginosa strain PAO1. The genome of PAO1 has been recently sequenced and can be used as a reference for compar isons of genetic organization in different strains. M13 clones that did not react with a DNA probe from PAO1 carried X24509-specific inserts. When a s imilar array hybridization analysis with DNA probes from different strains was used, a set of M13 clones which carried sequences present in the majori ty of human P. aeruginosa isolates from a wide range of clinical sources wa s identified, The inserts of these clones were used to identify cosmids enc ompassing a contiguous 48.9-kb region of the X24509 chromosome called PAGI- 1 (for "P. aeruginosa genomic island 1"). PAGI-1 is incorporated in the X24 509 chromosome at a locus that shows a deletion of a 6,729-bp region presen t in strain PAO1. Survey of the incidence of PAGI-1 revealed that this isla nd is present in 85% of the strains from clinical sources, Approximately ha lf of the PAGI-1-carrying strains show the same deletion as X24509, while t he remaining strains contain both the PAGI-1 sequences and the 6,729-bp PAO 1 segment, Sequence analysis of PAGI-1 revealed that it contains 51 predict ed open reading frames. Several of these genes encoded products with predic table function based on their sequence similarities to known genes, includi ng insertion sequences, determinants of regulatory proteins, a number of de hydrogenase gene homologs, and two for proteins of implicated in detoxifica tion of reactive oxygen species. It is very likely that PAGI-1 was acquired by a large number of P. aeruginosa isolates through horizontal gene transf er. The selection for its maintenance may be the consequence of expression of any one of the genes of unknown function or the genes which allow P. aer uginosa to survive under the conditions that generate reactive oxygen speci es. Alternatively, one or both of the transcriptional regulators encoded in PAGI-1 may control the expression of genes in the P. aeruginosa chromosome , which provides a selective advantage for strains that have acquired this genomic island.