Experimental evolution of enzyme temperature activity profile: Selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase

Authors
Roovers, H Sanchez, R Legrain, C Glansdorff, N
Citation
H. Roovers et al., Experimental evolution of enzyme temperature activity profile: Selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase, J BACT, 183(3), 2001, pp. 1101-1105
Citations number
25
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
3
Year of publication
2001
Pages
1101 - 1105
Database
ISI
SICI code
0021-9193(200102)183:3<1101:EEOETA>2.0.ZU;2-V
Abstract
We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransfer ase active at low temperatures by selecting for complementation of an appro priate yeast mutant after in vivo mutagenesis. The mutants,were double ones , still complementing at 15 degreesC, a temperature already in the psychrop hilic range. Their kinetic analysis is reported.