POSITION-INDEPENDENT AND ORIENTATION-INDEPENDENT ACTIVITY OF THE SCHIZOSACCHAROMYCES-POMBE MEIOTIC RECOMBINATION HOT-SPOT M26

Citation
Me. Fox et al., POSITION-INDEPENDENT AND ORIENTATION-INDEPENDENT ACTIVITY OF THE SCHIZOSACCHAROMYCES-POMBE MEIOTIC RECOMBINATION HOT-SPOT M26, Proceedings of the National Academy of Sciences of the United Statesof America, 94(14), 1997, pp. 7446-7451
Citations number
31
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
94
Issue
14
Year of publication
1997
Pages
7446 - 7451
Database
ISI
SICI code
0027-8424(1997)94:14<7446:PAOAOT>2.0.ZU;2-7
Abstract
The activity of the M26 meiotic recombination hot spot of Schizosaccha romyces pombe depends on the presence of the heptamer 5'-ATGACGT-3'. T ransplacement of DNA fragments containing the ade6-M26 gene to other c hromosomal loci has previously demonstrated that the heptamer function s in some, but not all, transplacements, suggesting that hot spot acti vity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1-4 bp in the ade6 and ural gen es, the heptamer was active as a recombination hot spot, in an orienta tion-independent manner, at all locations tested. Thus, the heptamer s equence can create an active hot spot in other chromosomal contexts, p rovided that the gross chromosomal structure is not altered; this resu lt is consistent with the hypothesis that a specific higher-order chro matin structure is required for M26 hot spot activity.