Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography

Authors
Li, HB Chen, F Zhang, TY Yang, FQ Xu, GQ
Citation
Hb. Li et al., Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography, J CHROMAT A, 905(1-2), 2001, pp. 151-155
Citations number
11
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
905
Issue
1-2
Year of publication
2001
Pages
151 - 155
Database
ISI
SICI code
Abstract
High-speed counter-current chromatography (HSCCC) was applied to the isolat ion and purification of lutein from microalgae. Analytical HSCCC was used f or the preliminary selection of a suitable solvent system composed of n-hex ane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. (C) 2001 Elsevier Science B .V. All rights reserved.