Applications of a copper microparticle-modified carbon fiber microdisk array electrode for the simultaneous determination of aminoglycoside antibiotics by capillary electrophoresis

A copper microparticle-modified carbon fiber microdisk array electrode was fabricated and employed in capillary electrophoresis for the simultaneous d etermination of the five aminoglycoside antibiotics (AGs) including netilmi cin, tobramycin, lincomycin, kanamycin and amikacin. The array electrode ex hibited high catalytic activity for AGs, good reproducibility and stability . Under the optimum separation conditions (separation voltage of 6.2 kV, el ectrophoretic medium of 125 mM NaOH), the five AGs above were baseline sepa rated within 20 min. At a working electrode potential of 0.7 V (versus satu rated calomel electrode), the calibration curves were linear over two order s of magnitude of concentration, and the detection limits (S/N = 3) were be low 2 muM except for lincomycin (6.7 muM) The developed method was successf ully employed for the simultaneous determination of the five AGs studied in pharmaceutical injections. The feasibility of this method for the simultan eous determination of lincomycin, kanamycin and amikacin in urine sample wa s also demonstrated. (C) 2001 Elsevier Science B.V. All rights reserved.