Isolation of the etiologic agent of human granulocytic ehrlichiosis from the 'white-footed mouse (Peromyscus leucopus)

Citation
Md. Ravyn et al., Isolation of the etiologic agent of human granulocytic ehrlichiosis from the 'white-footed mouse (Peromyscus leucopus), J CLIN MICR, 39(1), 2001, pp. 335-338
Citations number
25
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
1
Year of publication
2001
Pages
335 - 338
Database
ISI
SICI code
0095-1137(200101)39:1<335:IOTEAO>2.0.ZU;2-U
Abstract
We examined white-footed mice (Peromyscus leucopus) from Minnesota for infe ction with the etiologic agent of human granulocytic ehrlichiosis (HGE). Fr om April to September 1997, we collected P. leucopus from Washington County , Minnesota, an area enzootic for HGE. Blood was cultivated in HL60 cells f or isolation of the HGE agent. Of 59 mice examined, only a single mouse was culture positive for the HGE agent. The 16S ribosomal DNA sequence of the isolate was determined to be identical to that of the HGE agent. The isolat e was reactive with monoclonal antibodies to the 44-kDa antigen of the HGE agent and was infectious for laboratory mice.