Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene

Authors
Grigg, ME Boothroyd, JC
Citation
Me. Grigg et Jc. Boothroyd, Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene, J CLIN MICR, 39(1), 2001, pp. 398-400
Citations number
14
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
1
Year of publication
2001
Pages
398 - 400
Database
ISI
SICI code
0095-1137(200101)39:1<398:RIOVTI>2.0.ZU;2-8
Abstract
Sequence analysis at the 35-fold-repetitive B1 locus identified three restr iction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type I II strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical d isease in humans.