Rapid PCR-RFLP method for the identification of marine fish fillets (seabass, seabream, umbrine, and dentex)

A rapid and reliable PCR-RFLP method was optimized to identify marine fish fillets. Seabass, seabream, umbrine, and dentex were considered in the stud y. After DNA extraction and PCR, the 359 bp amplification products, obtaine d from gene encoding the cytochrome b, were subjected to restriction enzyme analysis, All the enzymes tested were not able to distinguish all the 5 sp ecies at the same time, but the combination of the results obtained from th e digestion HaeIII and NlaIII can be used to differentiate the fish fillets considered, The method described is sensitive, rapid, and reliable, and it could be used to expose fraudulent substitutions with less valuable fish.